Aarhus Universitets segl

CARMA - CARbon sequestration by Greenland’s Marine forests in a warming Arctic

Projektet afsluttedes 1. oktober 2023

Forskningsprojektet vil teste hypotesen om, at den andel af lagret kulstof på havbunden, som stammer fra Arktiske marine skove (tang og havgræsser) er stigende og af global relevans – med fokus på Grønland som test område.

Projektet er støttet af Danmarks Frie Forskningsfond (DFF) og ledet af forskere fra Aarhus universitet, Institut for Ecoscience og Institut for Geoscience i samarbejde med forskere fra King Abdullah University of Science and Technology (KAUST), Saudi Arabien, Helmholtz Zentrum Geesthacht, Institute of Coastal Research, Tyskland og Max Planck institute, Tyskland.

Final report

Rationale

Highly productive ecosystems on land and at sea provide natural carbon (C)-sinks. Loss of such sinks due to deforestation and land use changes have resulted in 32% of the accumulated global CO2 emissionsa,b. Marine forests, which contribute to oceanic C-sinks (blue C), are also under global threat due to multiple pressures. However, in the Arctic, these forests and their related C-sequestration may expand with warming and reduced sea-ice coverc. Macroalgal forests have typically been ignored as C-sinks, as they mainly grow on rocky shores where organic matter does not accumulate. Yet, macroalgae are C-donors to sinks beyond their habitat, and a first-order global estimate suggests that their contribution is substantiald. However, there are huge knowledge gaps in the distribution, production, and sequestration potential of macroalgae, especially in the vast and inaccessible Arctic.

Aim

The aim of CARMA was to test the main hypothesis that burial of C exported from Arctic marine forests provides a globally relevant, and growing, contribution to C-sequestration in marine sediments. The project posed three key questions:

  • Q1. Is C from marine forests present in Arctic marine sediments and, if so, what percent of the stock do they contribute?
  • Q2. Are there latitudinal and/or temporal trends in the contribution of marine forest to C stocks?
  • Q3. How much C do Greenland marine forests sequester?

Approach

CARMA applied an interdisciplinary approach at the intersection of marine ecology, geology, oceanography, remote sensing, biochemistry and genetics, with Greenland as a case study. We coupled conceptual advances with emerging techniques, in particular environmental DNA (eDNA) for tracing C from marine forests in Arctic marine sediments and used field observations, remote sensing and model approaches to identify the distribution of Arctic macroalgae, their potential C-export to oceanic sinks and to predict changes. To explore climate-related patterns in the contribution of marine vegetation of Arctic C-sinks, we analysed marine sediments and vegetation along Greenland/Arctic climate gradients, analysed deep sediment cores from the West Greenland coast as historical archives to explore long-term changes in fingerprints of macroalgae in relation to climate proxies, and explored trends in pan-Arctic marine vegetation. CARMA involved primary collaboration between excellent research environments in Denmark, Saudi Arabia, and Germany and expanded the collaboration to a larger international forum during the project period. This scientific environment made CARMA a unique framework for training young scientists.

Deviations

Developing the eDNA fingerprinting tools for macroalgae in marine sediments was more demanding than expected, and whereas metabarcoding methods proved successful to identify macroalgae to order-level, they did not allow proper quantification of macroalgal DNA. This requires quantitative polymerase chain reaction (qPCR) assays, which were developed by the end of the project for future use. Analyses of amino acid isotopes could also not be completed within the project period but are underway. The relative contribution of Arctic macroalgae to the pool of organic matter in the sediments was instead coarsely quantified based on stable isotopes of sediment organic matter and supplemented by top-down estimates of macroalgal distribution, trends, production, and associated estimates of potential export to C-sinks.
This latter approach was explored further than anticipated and, in addition to the Arctic angle, we engaged in a larger international collaboration to also develop global estimates of macroalgal distribution, production, potential contribution to C-sinks, and to evaluate opportunities and limitations in using macroalgae for climate change mitigation. We thereby reached goals beyond the initial aims and expanded our network.

Deviations

Marine vegetated habitats in the Arctic/Greenland: CARMA estimated the area distribution of marine vegetation at local- to Greenland- and pan-Arctic scale1-10. Local-scale distribution was assessed by remotely operated vehicle (ROV) and remote sensing (drones, satellites) for the vast Nuup Kangerlua fjord system in SW Greenland7 while species distribution models were used for assessing the potential distribution area of dense kelp forests (>50% cover) at the Nordic scale6 and of potential habitats of brown macroalgae and eelgrass at pan-Arctic scale9 and at global scale11.
For Greenland, the distribution area of dense kelp forests was modelled at 1304 km2 6. The modelled suitable habitat area of brown macroalgae was markedly larger and varied with modeling approach and resolution. The most updated estimate (based on stacked distribution modeling of individual species and corrected for % rocky coastline) was 11,007 km2 with an associated production of 362.9 g C m-2 y-1, totaling 4.0 TgCy-1 15. CARMA also identified recent increasing trends in pan-Arctic vegetation based on observational evidence supported by modeling9, and the same models predicted continued increases through this Century12.

Export of macroalgae to C-sinks: CARMA documented large-scale export of floating macroalgae from coastal habitats based on direct observations and satellite imagery. Tracking of export pathways from a large Greenland fjord system suggested that most of the exported algae was retained within the fjord with a fate of degradation, grazing8 or sequestration while a small fraction was exported offshore to potentially reach C-sinks in the deep sea7. Pathways of floating exported macroalgae off the coast of Greenland were further traced by GPS-marked buoys, satellites, and modeling in the Baffin Bay area, where novel vertical exchange patterns were documented3. CARMA also contributed to a global model of potential sequestration of macroalgal C in the deep sea based on modelled macroalgal production along the global coastline coupled to an ocean model tracking the export of macroalgae and on this basis estimating the transport time to reach beyond the shelf for potential sequestration in the deep sea and the likelihood that macroalgae would reach this sink before they were degraded. Overall, modelled global export of macroalgal POC beyond the shelf was 56 (1-170) TgCy-1 of which 4-44 TgC y-1 (1.1-11.8% of NPP) could be retained for >100 years15. For Greenland, the matching export of macroalgal POC beyond the shelf is 0.98 (0.28 - 2.6) TgC y-1 with 0.04-0.47 TgCy-1 potentially retained for >100 years15. These estimates do not include sequestration of macroalgal particles on the shelf or of dissolved C (DOC). Applying the 1st order estimate of total global macroalgal C-sequestration (avg. 11% of NPP)a yields 0.62 TgC for Greenland. These coarse estimates are our best reply to Q3.

Macroalgal blue carbon: We developed an eDNA fingerprinting tool to trace the contribution of marine forests to C-pools16 in sediments17-19. The tool consists of an 18S ribosomal DNA mini-barcode for marine macrophytes and an associated 18S reference library18,19, including a specific library and testing for key Arctic/North-Atlantic macroalgal species19. The tool documented macroalgal DNA in 94% of all (89) surface sediment samples distributed around Greenland and Svalbard from the shore to 1460 m depth and to 350 km offshore. The abundance of macroalgal sequences was highest near-shore and dominated by brown algae20. The tool also traced millennial-scale burial of macroalgae in six dated sediment cores distributed along a N-S gradient (79°N-60°N) on Greenland’s West Coast where especially brown macroalgal fingerprints were documented in 86.5% of all samples and dated 2600 years back21. The isotopic signal of the organic matter of surface sediments gave a coarse indication of a significant relative contribution of macroalgae to the pool of organic matter (range 21-85%)20. Overall, in reply to Q1, these results underline the prevalent presence of macroalgae in Greenland marine sediments and the potential for long-term storage. But better tools are needed for quantification. In reply to Q2, we did not identify latitudinal- or temporal trends in the contribution of macroalgae to C-pools.
In conclusion, CARMA advanced the knowledge on C-cycling of Arctic marine forests by

  1. providing the first quantitative estimates of the potential distribution of Arctic marine forests and their increasing trends,
  2. evidencing and documenting export pathways of Arctic macroalgae to potential C-sinks,
  3. documenting the prevalent presence of macroalgal C in Arctic surface sediments and millennial-scale preservation of macroalgal C in deeper sediments via new eDNA fingerprinting methods and
  4. providing a coarse estimate of potential C-sequestration by Greenland’s marine forests.

These findings partly supported the main hypothesis. Better quantification of the contribution of Arctic macroalgae to C-sequestration is, however, still needed. Although Arctic macroalgae contribute to C-sequestration, increases in their C-burial due to climate change is not eligible as a climate change mitigation strategy, which requires that the increase is linked to management, but the findings can guide the management of these important habitats.

Areas for further research

The data, concepts and tools developed by CARMA can be taken to the next level: Upscaling of mapping of tidal- and floating macroalgae to Greenland scale; refinement of macroalgal species distribution models and predictions, better quantification of macroalgal DNA by qPCR through sediment cores to quantify changes over time and experimentally testing links between macroalgal DNA and OC during degradation. Sequestration of macroalgal DOC is also a major unknown.

CARMA refs., incl. citations above

Other: aCiais et al. 2013. Cambridge Univ. Press., bArneth et al. 2017 Nature Geosci., cKrause-Jensen & Duarte 2014. Frontiers, dKrause-Jensen & Duarte 2016. Nature Geosci.

Publikationer

2023

  • Ross, F.W., Boyd, P.W., Filbee-Dexter, K., Watanabe, K., Ortega, A., Krause-Jensen, D., Lovelock, C., Sondak, C.F., Bach, L.T., Duarte, C.M. and Serrano, O., 2023. Potential role of seaweeds in climate change mitigation. Science of the Total Environment, p.163699. doi.org/10.1016/j.scitotenv.2023.163699
  • Ørberg SB, Duarte CM, Geraldi NR, Sejr M, Wegeberg S, Hansen JLS, Krause-Jensen D. 2023. Prevalent Fingerprint of Marine Macroalgae in Arctic surface sediments. Science of the Total Environment: 898. https://doi.org/10.1016/j.scitotenv.2023.165507
  • Filbee-Dexter, K., Pessarrodona, A., Duarte, C. M., Krause-Jensen, D., Hancke, K., Smale, D., & Wernberg, T. (2023). Seaweed forests are carbon sinks that may help mitigate CO2 emissions: a comment on Gallagher et al.(2022). ICES Journal of Marine Science, fsad107. https://doi.org/10.1093/icesjms/fsad107
  • Carlson, D. F., Vivó-Pons, A., Treier, U. A., Mätzler, E., Meire, L., Sejr, M., & Krause-Jensen, D. (2023). Mapping intertidal macrophytes in fjords in Southwest Greenland using Sentinel-2 imagery. Science of the Total Environment865, 161213. https://doi.org/10.1016/j.scitotenv.2022.161213
  • Ager, T. G., Krause-Jensen, D., Olesen, B., Carlson, D. F., Winding, M. H. S., & Sejr, M. K. (2023). Macroalgal habitats support a sustained flux of floating biomass but limited carbon export beyond a Greenland fjord. Science of The Total Environment, 162224. https://doi.org/10.1016/j.scitotenv.2023.162224

2022

  • Krause-Jensen, Dorte, Hege Gundersen, Mats Björk, Martin Gullström, Martin Dahl, Maria E. Asplund, Christoffer Boström et al. "Nordic blue carbon ecosystems: status and outlook." Frontiers in Marine Science 9 (2022). https://doi.org/10.3389/fmars.2022.847544
  • Fragkopoulou, E., Serrão, E. A., De Clerck, O., Costello, M. J., Araújo, M. B., Duarte, C. M., ... & Assis, J. (2022). Global biodiversity patterns of marine forests of brown macroalgae. Global Ecology and Biogeography31(4), 636-648. https://doi.org/10.1111/geb.13450
  • Assis, J., Serrão, E. A., Duarte, C. M., Fragkopoulou, E., & Krause-Jensen, D. (2022). Major expansion of marine forests in a warmer Arctic. Frontiers in Marine Science 9, 850368 https://doi.org/10.3389/fmars.2022.850368
  • Kvile, K. Ø., Andersen, G. S., Baden, S. P., Bekkby, T., Bruhn, A., Geertz-Hansen, O., ... & Gundersen, H. (2022). Kelp forest distribution in the Nordic region. Frontiers in Marine Science, 887.  https://doi.org/10.3389/fmars.2022.850359
  • Bringloe, T. T., Fort, A., Inaba, M., Sulpice, R., Ghriofa, C. N., MolsMortensen, A., ... & Verbruggen, H. (2022). Whole genome population structure of North Atlantic kelp confirms high‐latitude glacial refugia. Molecular Ecology31: 6473-6488. https://doi.org/10.1111/mec.16714
  • Duarte, C. M.Gattuso, J.-P.Hancke, K.Gundersen, H.Filbee-Dexter, K.Pedersen, M. F.Middelburg, J. J.Burrows, M. T.Krumhansl, K. A.Wernberg, T.Moore, P.Pessarrodona, A.Ørberg, S. B.Pinto, I. S.Assis, J.Queirós, A. M.Smale, D. A.Bekkby, T.Serrão, E. A., & Krause-Jensen, D. (2022). Global estimates of the extent and production of macroalgal forestsGlobal Ecology and Biogeography001– 18https://doi.org/10.1111/geb.13515

2021

2020

  • Krause-Jensen, D., Archambault, P., Assis, J., Bartsch, I., Bischof, K., Filbee-Dexter, K., ... & Duarte, C. M. (2020). Imprint of climate change on pan-Arctic marine vegetation. Frontiers in Marine Science, 7, 1-27. https://doi.org/10.3389/fmars.2020.617324
  • Ortega, A., Geraldi, N. R., Rua, R. D., Ørberg, S. B., Wesselmann, M., Krause-Jensen, D. & Duarte, C. M. (2020a). A DNA mini-barcode for marine macrophytes. Mol Ecol Resources, 20, 920–935. https://doi/10.1111/1755-0998.13164

2019

  • Macreadie PI, Anton A, Raven JA, Beaumont N, Connolly RM, Friess DA, Kelleway JJ, Kennedy H, Kuwae T,
Lavery PS, Lovelock CE, Smale DA, Apostolaki ET, Atwood TB, Baldock J, Bianchi TS, Chmura GL, Eyre BD, Fourqurean JW, Hall-Spencer JM, Huxham M, Hendriks IE, Krause-Jensen D, Laffoley D, Luisetti T, Marbà N, Masque P, McGlathery KJ, Megonigal JP, Murdiyarso D, Russell BD, Santos R, Serrano O, Silliman BR, Watanabe K, Duarte CM. 2019. The Future of Blue Carbon Science. Nature Communications 10: 3998. doi.org/10.1038/s41467-019-11693-w (open access) (open access)
  • Ortega A, Geraldi NR, Alam I, Kamau AA, Acinas SG, Logares R, Gasol JM, Massana R, Krause-Jensen D, Duarte CM. 2019. Important contribution of macroalgae to oceanic carbon sequestration. Nature Geoscience. Doi: 10.1038/s41561-019-0421-8.
  • Krause-Jensen D, Sejr M, Bruhn A, Rasmussen MB, Christensen PB, Hansen JLS, Duarte CM, Bruntse G, Wegeberg S. 2019. Deep penetration of kelps offshore along the west coast of Greenland. Frontiers in Marine Science 6:375. doi: 10.3389/fmars.2019.00375 (open access)
  • Queirós AM, Stephens N, Widdicombe S, Tait K, McCoy SJ, Ingels J, Rühl S, Airs R, Beesley A, Carnovale G, Cazenave1 P, Dashfield S, Hua E, Jones M, Lindeque P, McNeill CL, Nunes J, Parry H, Pascoe C, Widdicombe C, Smyth T, Atkinson A, Krause-Jensen D, Somerfield PJ. 2019. Connected macroalgal-sediment systems: blue carbon and foodwebs in the deep coastal ocean. Ecological Monographs, e01366. (open access)
  • Saderne, V., P. I. Macreadie, D. T. Maher, J. J. Middelburg, O. Serrano, H. Almahasheer, A. Arias-Ortiz, M. Cusack, B. D. Eyre, J. Fourqurean, H. Kennedy, D. Krause-Jensen, T. Kuwae, P. Lavery, C. E. Lovelock, N. Marba, P. Masqué, M. A. Mateo, I. Mazarrasa, K. J. McGlathery, M. P. J. Oreska, C. J. Sanders, I. R. Santos, J. M. Smoak, T. Tanaya, K. Watanabe, C. M. Duarte. 2019. Role of carbonate burial in “Blue Carbon” budgets. Nature communications. https://doi.org/10.1038/s41467-019-08842-6. (open access)
  • Sanz-Martín M, Hendriks IE, Carstensen J, Marba N, Krause-Jensen D, Sejr MK, Duarte CM. 2019. Continuous photoperiod of the Artic summer stimulates the photosynthetic response of some marine macrophytes. Aquatic Botany 158: 103126. https://doi.org/10.1016/j.aquabot.2019.06.005

2018

Andre udgivelser

2023

  • Ørberg, S., Duarte, C., Geraldi, N., Sejr, M., Wegeberg, S., Hansen, J., Krause-Jensen, D. (2023). Tracing macroalgae in Arctic sediments using environmental DNA. P. 28-30 in Christensen T.R. & Arndal M.F. GEM Annual report Card 2022. Zenodo. https://doi.org/10.5281/zenodo.8141553 DCE – Danish Centre for Environment and Energy, Aarhus University, 52 pp.

2021

  • Krause-Jensen, D and Duarte, CM 2021. Climate change and the expansion of marine forests around Greenland’s coast. Extreme-E Global Legacy Workshop. Presentation at Kangerlussuaq 28. August 2021. (Presented by CM Duarte) https://www.extreme-e.com/en/legacy-projects/greenland
  • Krause-Jensen D. 2021. “Wild Macroalgae” Presentation at the virtual conference “Blue Carbon – a new frontier for conservation? Building ambition ahead of COP26” organized by the Blue Marine Foundation, 9. June 2021. (Direct link to my presentation: https://www.youtube.com/watch?v=N1FSCDRQJ_o&t=29s; Direct link to online conference: https://www.youtube.com/watch?v=Ibg59z7Ua48&t=8s)
  • Ørberg, S. 2021. Fingerprint of Marine Forests in Arctic surface sediments. ASLO Aquatic Sciences meeting, On-line, June 22-27, 2021
  • Krause-Jensen D. 2021. Marine forests and blue carbon. Lecture Series 2021 - Department of Biology, University of Southern Denmark, On-line, February 5, 2021
  • Krause-Jensen, D. 2021. Fate of macroalgae in the natural habitat. The National Academies of Science, Engineering and Medicine’s (NASEM)  Workshop on Ocean-based CDR Opportunities and Challenges - Part 3: Ecosystem Recovery & Seaweed Cultivation. Links: A Research Strategy for Ocean Based Carbon Dioxide Removal and Sequestration, project website Board, you can sign up for our listserv here (in the “Connect with Us” box).   
  • Krause-Jensen D. 2021. ‘Changing seaweed along Greenland’s coasts and beyond and Blue Carbon perspectives’ ARCTOS on-line Colloquium “Macroalgae of high latitudes. 19.-20. January 2021.

2020

2019

  • Krause-Jensen D & Duarte CM. Coordination of the Euromarine foresight workshop: “The role of macroalgae in the global carbon cycle”, Carmen de la Victoria, Granada, April 7-10, 2019.
  • Krause-Jensen D & Duarte C. 2019. Macroalgae in the Blue Carbon context – including seaweed farming perspectives. Presentation at Blue Carbon International workshop in Copenhagen 9-13. September 2019.
  • Krause-Jensen D & Holmer M. 2019. Blue Forests in Denmark and Greenland – Status and knowledge gaps. Presentation at Blue Carbon International workshop in Copenhagen 9-13. September 2019.
  • Krause-Jensen D. 2019. Surveys of kelp and tidal algae in Greenland. Presentation at MARFOR (Marine forests) workshop, Faro, 27-28 February 2019.
  • Krause-Jensen D. 2019. The elephant in the blue carbon room - the role of macroalgae: status and aims. Final workshop for the Nordic blue carbon project, 21-22 November 2019, Miljødirektoratet, Helsfyr, Oslo
  • Krause-Jensen D. 2019. Nordic blue forests- Status, possibilities and challenges. Nordic ocean and climate workshop: Priorities for Nordic countries in International work. May 13-14 2019, Oslo.

Projektet er støttet af Danmarks Frie Forskningsfond (DFF). Dorte Krause-Jensen fra Aarhus Universitet, Institut for Ecoscience leder projektet i samarbejde med Carlos Duarte fra King Abdullah University of Science and Technology (KAUST), Saudi Arabien. Projektet foregår i tæt samarbejde med en kerne-projektgruppe bestående af Sarah Backmann Ørberg og Mikael Sejr fra Aarhus Universitet, Institut for Ecoscience, Marit-Solveig Seidenkrantz, Århus Universitet, Institut for Geoscience, Daniel Frazier Carlson Helmholtz Zentrum Geesthacht, Institute of Coastal Research, Tyskland og Thomas Larsen, og Max Planck institute, Tyskland. Udover kernegruppen deltager flere kollegaer og studerende også i projektet.

Samarbejdspartnere

Focus/header

Greenlandic marine forest of macroalgae (Sugar kelp). Photo: Scott Bennett ©